Database : HANSEN Search on : INCLUSAO EM PARAFINA [Subject descriptor] References found : 7 [refine] Displaying: 1 .. 7   in format [Detailed]

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Id: 17559 Author: Save, M. P; Shetty, V. P; Shetty, K. T; Antia, N. H Title: Alterations in neurofilament protein(s) in human leprous nerves: morphology, immunohistochemistry and Western immunoblot correlative study ..- Source: s.l; s.n; 2004. 16 p. ilus, tab. Abstract: Using a specific antibody (SMI 31), the state of phosphorylation of high and medium molecular weight neurofilaments (NF-H and NF-M) was studied in 22 leprous and four nonleprous human peripheral nerves by means of immunohistochemistry, sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and Western immunoblot (WB). The results thus obtained were compared with morphological changes in the respective nerves studied through light and electron microscopy. Many of the leprous nerves showing minimal pathology revealed lack of or weak staining with SMI 31, denoting dephosphorylation. Remyelinated fibres stained intensely with SMI 31 antibody. The WB analysis of Triton X-100 insoluble cytoskeletal preparation showed absence of regular SMI 31 reactive bands corresponding to 200 and 150 kDa molecular weight (NF-H and NF-M, respectively) in 10 nerves. Three of the 10 nerves revealed presence of NF protein bands in SDS-PAGE but not in WB. Presence of additional protein band (following NF-M) was seen in four nerves. Two nerves revealed NF-H band but not NF-M band and one nerve showed trace positivity. In the remaining five nerves presence of all the three NF bands was seen. Thus, 77.3% (17/22) of human leprous nerves studied showed abnormal phosphorylation of NF protein(s). The ultrastructural study showed abnormal compaction and arraying of NF at the periphery of the axons in the fibres with altered axon to myelin thickness ratio (atrophied fibres) as well as at the Schmidt-Lantermann (S-L) cleft region. Such NF changes were more pronounced in the severely atrophied axons suggesting a direct correlation. The observed well-spaced NF in the remyelinated fibres under ultrastructural study was in keeping with both intense SMI 31 staining and presence of NF triplet bands seen in WBs in four of leprous nerves that showed a large number of regenerating fibres suggesting reversal of changes with regeneration. Findings in the present study suggest that atrophy, that is, the reduction in axonal calibre and paranodal demyelination, seen in leprous nerves may result from dephosphorylation of NF-H and NF-M proteins. (AU). Descriptors: Doença de Alzheimer/PA Esclerose Amiotrófica Lateral/PA Atrofia Axônios/PA Western Blotting Citoesqueleto/ME/PA Doenças Desmielinizantes/PA Eletroforese em Gel de Poliacrilamida Imunohistoquímica Hanseníase/*ME Fibras Nervosas/PA Proteínas de Neurofilamentos/*ME Neurônios/*ME/*PA/UL Inclusão em Parafina Fosforilação Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09338/s

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Id: 17283 Author: Osaki, Mitsuhiko; Adachi, Hironobu; Gomyo, Yoshihito; Yoshida, Haruhiko; Ito, Hisao Title: Detection of mycobacterial DNA in formalin-fixed, paraffin-embedded tissue specimens by duplex polymerase chain reaction: application to histopathologic diagnosis ..- Source: s.l; s.n; 1997. 6 p. ilus, tab. Abstract: Granuloma is a chronic inflammatory process associated with noninfectious agents or infectious diseases such as tuberculosis. Determination of the causative agent might be occasionally difficult in histopathologic sections. In this study, we examined 60 specimens of granuloma or inflammatory lesions that were originally diagnosed as 51 cases of granulomatous inflammation, 6 of leprosy, and 3 of atypical mycobacteriosis. The diagnoses in the last two categories were made both histologically and clinically. All of the sections and DNA were prepared from formalin-fixed, paraffin-embedded blocks. Histopathologic and immunohistochemical findings were compared with the results of duplex polymerase chain reaction (PCR) using two primers to amplify mycobacterial-common 383-base pair (bp) DNA and Mycobacterium tuberculosis-complex-specific 240-bp DNA. Six samples of leprosy and three of atypical mycobacteriosis showed the 383-bp but not the 240-bp band. Among the 51 specimens of granulomatous inflammations, nine showed no band of even the beta-globin, the cases being excluded from this analysis. The 42 specimens of granulomatous inflammation were subdivided into three categories by PCR: (1) 383- and 240-bp positive; (2) 383-bp positive and 240-bp negative; and (3) both negative. Category 1 included 32 specimens (76.2%), being considered as tuberculosis. One specimen was classified into Category 2, indicating possible atypical mycobacterium. Category 3 included nine specimens, composed of five of sarcoidosis and four other agent-induced granulomas, when compared with histologic and clinical findings. These findings indicate that the PCR assay using DNA extracted from paraffin-embedded materials provides useful information to differentiate tuberculosis from other type of granulomas. (AU). Descriptors: Primers do DNA DNA Bacteriano/*AN Diagnóstico Diferencial Fixadores Formaldeído Granuloma/*DI Micobactérias Atípicas/GE Mycobacterium/*GE Mycobacterium leprae/GE Mycobacterium tuberculosis/GE Inclusão em Parafina Reação em Cadeia da Polimerase Tuberculose/*DI Limits: Humano Location: BR191.1; 09184/s

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Id: 13741 Author: Fiallo, Paolo; Clapasson, Andrea; Favre, Anna; Pesce, Carlo Title: Overexpression of vascular endothelial growth factor and its endothelial cell receptor KDR in type I leprosy reaction ..- Source: s.l; s.n; Feb. 2002. 6 p. ilus, tab. Abstract: The sites of expression of vascular endothelial growth factor (VEGF) and of KDR, its endothelial cell receptor, were investigated in leprosy reaction Type 1, or reversal reaction (RR), by immunohistochemistry and in situ hybridization. In comparison with nonreactional leprosy, overexpression of both VEGF and KDR was seen in granuloma cells, especially epithelioid and foreign body-type giant cells, the epithelium and the vascular endothelium of RR specimens. In granuloma cells, hybridization for VEGF was stronger than immunostaining, a finding that may reflect the rapid turnover of VEGF in an immunologically dynamic situation such as RR. In the epidermis, double immunohistochemistry revealed VEGF overexpression in CDla-positive dendritic cells. The VEGF may not only be relevant for hyperpermeability and mononuclear cell differentiation (the key morphologic features in the acute, clinically evident phase of RR), but it could also be implicated in RR onset, when dendritic cells are activated in response to antigen stimulation. (AU). Descriptors: PRIMERS DO DNA FATORES DE CRESCIMENTO ENDOTELIAL/metab HANSENIASE DIMORFA/metab HANSENIASE DIMORFA/patol RECEPTORES DOS FATORES DE CRESCIMENTO/metab RECEPTORES MITOGÊNICOS/metab LINFOCINAS/metab RECEPTORES PROTEINA TIROSINA QUINASES/metab IMUNOHISTOQUIMICA HIBRIDIZACAO IN SITU - INCLUSAO EM PARAFINA Limits: HUMANO SUPPORT, NON-U.S. GOV'T RECEPTORES DE FATOR DE CRESCIMENTO DO ENDOTELIO VASCULAR Electronic Medium: http://www.ilsl.br Location: BR191.1; 09033/s

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Id: 11947 Author: Fiallo, Paolo; Clapassom, Andrea; Favre, Anna; Pesce, Carlo Title: Overexpression of vascular endothelial growth factor and its endothelial cell receptor KDR in type 1 leprosy reaction ..- Source: s.l; s.n; 2002. 6 p. ilus, tab. Descriptors: HANSENIASE DIMORFA HANSENIASE DIMORFA PRIMERS DO DNA FATORES DE CRESCIMENTO ENDOTELIAL IMUNOHISTOQUIMICA HIBRIDIZAÇAO IN SITU LINFOCINAS INCLUSAO EM PARAFINA RECEPTORES DOS FATORES DE CRESCIMENTO RECEPTORES MITOGENICOS Location: BR191.1; 08598/s

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Id: 11729 Author: Nishimura, Masayuki; Kwon, Kyung Sool; Shibuta, Kenji; Yoshikawa, Yasuji; Oh, Chang-Keun; Suzuki, Tomokazu; Chung, Tae-Ahn; Hori, Yoshiaki Title: An improved method for DNA diagnosis of leprosy using formaldehyde-fixed, paraffin-embedded skin biopsies ..- Source: s.l; s.n; 1994. 4 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES DNA BACTERIANO ELETROFORESE EM GEL DE AGAR FORMALDEIDO DADOS DE SEQUENCIA MOLECULAR INCLUSAO EM PARAFINA REAÇAO EM CADEIA POR POLIMERASE SEQUENCIAS REGULADORAS DE ACIDO NUCLÉICO SENSIBILIDADE E ESPECIFICIDADE PELE FIXAÇAO DE TECIDOS Location: BR191.1; 07218/s

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Id: 10260 Author: Sung, Kyung Jeh; Kim, Sung Bum; Choi, Jee Ho; Koh, Kyoung; Na, Doe Sun Title: Detection of mycobacterium leprae DNA in formalin-fixed paraffin-embedded samples from multibacillary and paucibacillary leprosy patients by polymerase chain reaction ..- Source: s.l; s.n; 1993. 4 p. ilus. Descriptors: SOUTHERN BLOTTING DNA BACTERIANO DNA BACTERIANO FORMALDEIDO AMPLIFICAÇAO DE GENES HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE INCLUSAO EM PARAFINA REAÇAO EM CADEIA POR POLIMERASE SENSIBILIDADE E ESPECIFICIDADE FIXAÇAO DE TECIDOS Location: BR191.1; 06939/s

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Id: 10163 Author: Miko, T. L; Gschmeissner, S. E Title: Histological methods for assessing myelin sheaths and axons in human nerve trunks ..- Source: s.l; s.n; 1994. 10 p. ilus. Descriptors: AXONIOS CRIOPRESERVAÇAO HEMATOXILINA HISTOCITOQUIMICA PROCESSAMENTO DE IMAGENS ASSISTIDA POR COMPUTADOR HANSENIASE HANSENIASE BAINHA DE MIELINA FIBRAS NERVOSAS MIELINIZADAS TETROXIDO DE OSMIO INCLUSAO EM PARAFINA NERVOS PERIFÉRICOS IMPREGNAÇAO PELA PRATA NERVO TIBIAL NERVO TIBIAL FERIMENTOS E LESOES FERIMENTOS E LESOES Location: BR191.1; 07076/s

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